Bottlenecks of Molecular Mass Determination of Oligomeric Membrane Protein - Detergent Complexes and What can be Achieved

Bottlenecks of Molecular Mass Determination of Oligomeric Membrane Protein - Detergent Complexes and What can be Achieved

Avijit Pramanik1 and Sanjay Kumar2,3* 1Department of Microbiology, Central University of Haryana, Jant – Pali, Mahendergarh, India – 123031 2Department of Biochemistry, Central University of Haryana, Jant – Pali, Mahendergarh, India – 123031 3Burnett School of Biomedical Sciences, University of Central Florida, Orlando, USA *Corresponding author: Sanjay Kumar, Assistant Professor and Head, Depa...

Institutional Bottlenecks: What Can be Done?*

Determination of molecular weight of the protein moiety in protein-detergent complexes without direct knowledge of detergent binding.

Sedimentation equilibrium measurements can be used to determine the molecular weight of the protein moiety of a protein-detergent complex without prior knowledge of detergent binding. The procedure is to adjust the solvent density by addition of D2O so as to blank out the contribution of bound detergent to the sedimentation potential. An approximate measure of detergent binding can be obtained ...

architecture and engineering of nanoscale sculptured thin films and determination of their properties

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